Kusol Pootanakit

LOCUS       Kusol Pootanakit               16805 bp ds-DNA     circular SYN 16-MAY-2022
DEFINITION  Nuclease Cpf1 adapted for Aspergillus species.
ACCESSION   .
VERSION     .
KEYWORDS    pCRISPR01-FnCpf1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16805)
  AUTHORS   Abdulrachman D, Eurwilaichitr L, Champreda V, Chantasingh D, 
            Pootanakit K
  TITLE     Development of a CRISPR/Cpf1 system for targeted gene disruption in 
            Aspergillus aculeatus TBRC 277.
  JOURNAL   BMC Biotechnol. 2021 Feb 11;21(1):15. doi: 
            10.1186/s12896-021-00669-8.
  PUBMED    33573639
REFERENCE   2  (bases 1 to 16805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 16, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..16805
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(63..96)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(566..582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(681..698)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(852..1440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(932..951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1562..2422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2185..2204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2423..2514)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    2807..2840
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      3153..8403
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     primer_bind     8664..8680
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8664..8680
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8694..8713)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8695..8713)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8720..8736)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8729..8751)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(8943..9752)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             14811..14831
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             15665..16039
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 aggtaaatcc ccactaccgc attaagacct cagcgcggcc gcaaatttaa ataaaatgaa
       61 gtgaagttcc tatactttct agagaatagg aacttctata gtgagtcgaa taagggcgac
      121 acaaaattta ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt
      181 ttgtattatc gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt
      241 tcgagattta ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc
      301 ataaataata gatgaatagt ttaattatag gtgttcatca atcaaaaaag caacgtatct
      361 tatttaaagt gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc
      421 aaagtgacag gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa
      481 aaaaacccgc cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga
      541 accgcccagg gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt
      601 agaaacgcaa aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc
      661 ctactctcgc cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      721 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      781 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      841 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      901 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      961 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1021 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1081 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1141 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1201 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1261 aagtggtggg ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1321 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1381 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1441 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact
     1501 cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg
     1561 cttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1621 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1681 ccagcgctgc gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa
     1741 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1801 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1861 acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1921 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1981 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2041 tcatggttat ggcagcgcta cataattctc ttactgtcat gccatccgta agatgctttt
     2101 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2161 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2221 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2281 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2341 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2401 cacggaaatg ttgaatactc atattcttcc tttttcaata ttattgaagc atttatcagg
     2461 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2521 tcagtgttac aaccaattaa ccaattctga acattatcgc gagcccattt atacctgaat
     2581 atggctcata acaccccttg tttgcctggc ggcagtagcg cggtggtccc acctgacccc
     2641 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggac tccccatgcg
     2701 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2761 tcgcccgggc taattatggg gtgtcgccct tattcgactc tatagtgaag ttcctattct
     2821 ctagaaagta taggaacttc tgaagtgggg atttaaatgc ggccgcgctg agggtttaat
     2881 cgacgaagca gctgacggcc agtgccaagc ttaacgcgta ccgggcccag tatatgttcc
     2941 gcagatgact ggagctctgc catacgtgcc ctctcaagca ccatttgttc catctacaga
     3001 gactagtcac caactagtct atcaagactc acagggtaca ttgctgagac caactgacca
     3061 gaggcagggt agcggattga cggctccatc tccttcactt acaaggtcta ttgaaagccc
     3121 tttagcatca ccaagcggag aatagattgt taagcttatt ttttgtatac tgttttgtga
     3181 tagcacgaag tttttccacg gtatcttgtt aaaaatatat atttgtggcg ggcttaccta
     3241 catcaaatta ataagagact aattataaac taaacacaca agcaagctac tttagggtaa
     3301 aagtttataa atgcttttga cgtataaacg ttgcttgtat ttattattac aattaaaggt
     3361 ggatagaaaa cctagagact agttagaaac taatctcagg tttgcgttaa actaaatcag
     3421 agcccgagag gttaacagaa cctagaaggg gactagatat ccgggtaggg aaacaaaaaa
     3481 aaaaaacaag acagccacat attagggaga ctagttagaa gctagttcca ggactaggaa
     3541 aataaaagac aatgatacca cagtctagtt gacaactaga tagattctag attgaggcca
     3601 aagtctctga gatccaggtt agttgcaact aatactagtt agtatctagt ctcctataac
     3661 tctgaagcta gaataactta ctactattat cctcaccact gttcagctgc gcaaacggag
     3721 tgattgcaag gtgttcagag actagttatt gactagtcag tgactagcaa taactaacaa
     3781 ggtattaacc taccatgtct gccatcaccc tgcacttcct cgggctcagc agccttttcc
     3841 tcctcatttt catgctcatt ttccttgttt aagactgtga ctagtcaaag actagtccag
     3901 aaccacaaag gagaaatgtc ttaccacttt cttcattgct tgtctctttt gcattatcca
     3961 tgtctgcaac tagttagagt ctagttagtg actagtccga cgaggacttg cttgtctccg
     4021 gattgttgga ggaactctcc agggcctcaa gatccacaac agagccttct agaagactgg
     4081 tcaataacta gttggtcttt gtctgagtct gacttacgag gttgcatact cgctcccttt
     4141 gcctcgtcaa tcgatgagaa aaagcgccaa aactcgcaat atggctttga accacacggt
     4201 gctgagacta gttagaatct agtcccaaac tagcttggat agcttacctt tgccctttgc
     4261 gttgcgacag gtcttgcagg gtatggttcc tttctcacca gctgatttag ctgccttgct
     4321 accctcacgg cggatctgca taaagagtgg ctagaggtta taaattagca ctgatcctag
     4381 gtacggggct gaatgtaact tgccyttcct ttctcatcgc gcggcaagac aggcttgctc
     4441 aaattcctac cagtcacagg ggtatgcacg gcgtacggac cacttgaact agtcacagat
     4501 tagttagcaa ctagtctgca ttgaatggct gtacttacgg gccctcgcca ttgtcctgat
     4561 catttccagc ttcaccctcg ttgctgcaaa gtagttagtg actagtcaag gactagttga
     4621 aatgggagaa gaaactcacg aattctcgac acccttagta ttgtggtcct tggacttggt
     4681 gctgctatat attagctaat acactagtta gactcacaga aacttacgca gctcgcttgc
     4741 gcttcttggt aggagtcggg gttgggagaa cagtgccttc aaacaagcct tcataccatg
     4801 ctacttgact agtcagggac tagtcaccaa gtaatctaga taggacttgc ctttggcctc
     4861 catcagttcc ttcatagtgg gaggtccatt gtgcaatgta aactccatgc cgtgggagtt
     4921 cttgtccttc aagtgcttga ccaatatgtt tctgttggca gagggaacct gtcaactagt
     4981 taataactag tcagaaacta gtatagcagt agactcactg tacgcttgag gcatcccttc
     5041 actcggcagt agacttcata tggatggata tcaggcacgc cattgtcgtc ctgtggacta
     5101 gtcagtaact aggcttaaag ctagtcgggt cggcttacta tcttgaaatc cggcagcgta
     5161 agctccccgt ccttaactgc ctcgagatag tgacagtact ctggggactt tcggagatcg
     5221 ttatcgcgaa tgctcggcat actaatcgtt gactagtctt ggactagtcc cgagcaaaaa
     5281 ggattggagg aggaggagga aggtgagagt gagacaaaga gcgaaataag agcttcaaag
     5341 gctatctcta agcagtatga aggttaagta tctagttctt gactagattt aaaagagatt
     5401 tcgactagtt atgtacctgg agtttggata taggaatgtg ttgtggtaac gaaatgtaag
     5461 ggggaggaaa gaaaaagtcg gtcaagaggt aactctaagt cggccattcc tttttgggag
     5521 gcgctaacca taaacggcat ggtcgactta gagttagctc agggaattta gggagttatc
     5581 tgcgaccacc gaggaacggc ggaatgccaa agaatcccga tggagctcta gctggcggtt
     5641 gacaacccca ccttttggcg tttctgcggc gttgcaggcg ggactggata cttcgtagaa
     5701 ccagaaaggc aaggcagaac gcgctcagca agagtgttgg aagtgatagc atgatgtgcc
     5761 ttgttaacta ggtcaaaatc tgcagtatgc ttgatgttat ccgaagtgtg agagaggaag
     5821 gtccaaacat acacgattgg gagagggcct aggtataaga gtttttgagt agaacgcatg
     5881 tgagcccagc catctcgagg agattaaaca cgggccggca tttgatggct atgttagtac
     5941 cccaatggaa acggtgagag tccagtggtc gcagataact ccctaaattc cctgagctaa
     6001 ctctaagtcg accatgccgt ttatggttag cgcctcccaa aaaggaatgg ccgacttaga
     6061 gttacctctt gaccgacttt ttctttcctc ccccttacat ttcgttacca caacacattc
     6121 ctatatccaa actccaggta cataactagt cgaaatctct tttaaatcta gtcaagaact
     6181 agatacttaa ccttcatact gcttagagat agcctttgaa gctcttattt cgctctttgt
     6241 ctcactctca ccttcctcct cctcctccaa tcctttttgc tcgggactag tccaagacta
     6301 gtcaacgatt agtatgccga gcattcgcga taacgatctc cgaaagtccc cagagtactg
     6361 tcactatctc gaggcagtta aggacgggga gcttacgctg ccggatttca agatagtaag
     6421 ccgacccgac tagctttaag cctagttact gactagtcca caggacgaca atggcgtgcc
     6481 tgatatccat ccatatgaag tctactgccg agtgaaggga tgcctcaagc gtacagtgag
     6541 tctactgcta tactagtttc tgactagtta ttaactagtt gacaggttcc ctctgccaac
     6601 agaaacatat tggtcaagca cttgaaggac aagaactccc acggcatgga gtttacattg
     6661 cacaatggac ctcccactat gaaggaactg atggaggcca aaggcaagtc ctatctagat
     6721 tacttggtga ctagtccctg actagtcaag tagcatggta tgaaggcttg tttgaaggca
     6781 ctgttctccc aaccccgact cctaccaaga agcgcaagcg agctgcgtaa gtttctgtga
     6841 gtctaactag tgtattagct aatatatagc agcaccaagt ccaaggacca caatactaag
     6901 ggtgtcgaga attcgtgagt ttcttctccc atttcaacta gtccttgact agtcactaac
     6961 tactttgcag caacgagggt gaagctggaa atgatcagga caatggcgag ggcccgtaag
     7021 tacagccatt caatgcagac tagttgctaa ctaatctgtg actagttcaa gtggtccgta
     7081 cgccgtgcat acccctgtga ctggtaggaa tttgagcaag cctgtcttgc cgcgcgatga
     7141 gaaaggaarg gcaagttaca ttcagccccg tacctaggat cagtgctaat ttataacctc
     7201 tagccactct ttatgcagat ccgccgtgag ggtagcaagg cagctaaatc agctggtgag
     7261 aaaggaacca taccctgcaa gacctgtcgc aacgcaaagg gcaaaggtaa gctatccaag
     7321 ctagtttggg actagattct aactagtctc agcaccgtgt ggttcaaagc catattgcga
     7381 gttttggcgc tttttctcat cgattgacga ggcaaaggga gcgagtatgc aacctcgtaa
     7441 gtcagactca gacaaagacc aactagttat tgaccagtct tctagaaggc tctgttgtgg
     7501 atcttgaggc cctggagagt tcctccaaca atccggagac aagcaagtcc tcgtcggact
     7561 agtcactaac tagactctaa ctagttgcag acatggataa tgcaaaagag acaagcaatg
     7621 aagaaagtgg taagacattt ctcctttgtg gttctggact agtctttgac tagtcacagt
     7681 cttaaacaag gaaaatgagc atgaaaatga ggaggaaaag gctgctgagc ccgaggaagt
     7741 gcagggtgat ggcagacatg gtaggttaat accttgttag ttattgctag tcactgacta
     7801 gtcaataact agtctctgaa caccttgcaa tcactccgtt tgcgcagctg aacagtggtg
     7861 aggataatag tagtaagtta ttctagcttc agagttatag gagactagat actaactagt
     7921 attagttgca actaacctgg atctcagaga ctttggcctc aatctagaat ctatctagtt
     7981 gtcaactaga ctgtggtatc attgtctttt attttcctag tcctggaact agcttctaac
     8041 tagtctccct aatatgtggc tgtcttgttt tttttttttt tgtttcccta cccggatatc
     8101 tagtcccctt ctaggttctg ttaacctctc gggctctgat ttagtttaac gcaaacctga
     8161 gattagtttc taactagtct ctaggttttc tatccacctt taattgtaat aataaataca
     8221 agcaacgttt atacgtcaaa agcatttata aacttttacc ctaaagtagc ttgcttgtgt
     8281 gtttagttta taattagtct cttattaatt tgatgtaggt aagcccgcca caaatatata
     8341 tttttaacaa gataccgtgg aaaaacttcg tgctatcaca aaacagtata caaaaaataa
     8401 gcttaacaat ctattctccg cttggtgatg ctaaagggct ttcaatagac cttgtaagtg
     8461 aaggagatgg agccgtcaat ccgctaccct gcctctggtc agttggtctc agcaatgtac
     8521 cctgtgagtc ttgatagact agttggtgac tagtctctgt agatggaaca aatggtgctt
     8581 gagagggcac gtatggcaga gctccagtca tctgcggaac atatactggg cccgggaaga
     8641 tccgatatcg ccgtggcggc cgctctagaa ctagtggatc gatccccaat tcgccctata
     8701 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8761 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8821 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgagctt
     8881 gcgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct
     8941 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     9001 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     9061 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     9121 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     9181 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
     9241 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     9301 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     9361 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     9421 tattcttcta atacctggaa tgctgttttt ccggggatcg cagtggtgag taaccatgca
     9481 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     9541 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     9601 aacaactctg gcgcatcggg cttcccatac aagcgataga ttgtcgcacc tgattgcccg
     9661 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     9721 ggcctcgacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     9781 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     9841 ttttgagaca caacgtggct ttcccccccc cccctcgact ctaggataat ctcatgacca
     9901 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccttaata agatgatctt
     9961 cagatctcat ggtcatagct gtttccgctg agggtttaat taagacctca gccgagacag
    10021 cagaatcacc gcccaagtta agcctttgtg ctgatcatgc tctcgaacgg gccaagttcg
    10081 ggaaaagcaa aggagcgttt agtgaggggc aatttgactc acctcccagg caacagatga
    10141 ggggggcaaa aagaaagaaa ttttcgtgag tcaatatgga ttccgagcat cattttcttg
    10201 cggtctatct tgctacgtat gttgatcttg acgctgtgga tcaagcaacg ccactcgctc
    10261 gctccatcgc aggctggtcg cagacaaatt aaaaggcggc aaactcgtac agccgcgggg
    10321 ttgtccgctg caaagtacag agtgataaaa gccgccatgc gaccatcaac gcgttgatgc
    10381 ccagcttttt cgatccgaga atccaccgta gaggcgatag caagtaaaga aaagctaaac
    10441 aaaaaaaaat ttctgcccct aagccatgaa aacgagatgg ggtggagcag aaccaaggaa
    10501 agagtcgcgc tgggctgccg ttccggaagg tgttgtaaag gctcgacgcc caaggtggga
    10561 gtctaggaga agaatttgca tcgggagtgg ggcgggttac ccctccatat ccaatgacag
    10621 atatctacca gccaagggtt tgagcccgcc cgcttagtcg tcgtcctcgc ttgcccctcc
    10681 ataaaaggat ttcccctccc cctcccacaa aattttcttt cccttcctct ccttgtccgc
    10741 ttcagtacgt atatcttccc ttccctcgct tctctcctcc atccttcttt catccatctc
    10801 ctgctaactt ctctgctcag cacctctacg cattactagc cgtagtatct gagcacttct
    10861 cccttttata ttccacaaaa cataacacaa ccttcaccat ggccatctac caggagttcg
    10921 tcaacaagta cagcctgtcc aagaccctgc gcttcgagct gatcccgcag ggcaagaccc
    10981 tggagaacat caaggctcgc ggcctgatcc tggacgacga gaagcgcgcc aaggactaca
    11041 agaaggccaa gcagatcatc gacaagtacc accagttctt catcgaggag atcctgagca
    11101 gcgtctgcat ctcggaggac ctgctccaga actacagcga cgtgtacttc aagctgaaga
    11161 agtcggacga cgacaacctc cagaaggact tcaagagcgc caaggacacc atcaagaagc
    11221 agatcagcga gtacatcaag gactccgaga agttcaagaa cctgttcaac cagaacctga
    11281 tcgacgccaa gaagggccag gagtccgacc tgatcctgtg gctgaagcag tcgaaggaca
    11341 acggcatcga gctgttcaag gccaactcgg acatcaccga catcgacgag gccctggaga
    11401 tcatcaagag cttcaagggc tggaccacct acttcaaggg cttccacgag aaccgcaaga
    11461 acgtctacag cagcaacgac atcccgacca gcatcatcta ccgcatcgtg gacgacaacc
    11521 tgcccaagtt cctggagaac aaggccaagt acgagtccct gaaggacaag gcccccgagg
    11581 ccatcaacta cgagcagatc aagaaggacc tggccgagga gctgaccttc gacatcgact
    11641 acaagacctc cgaggtcaac cagcgcgtgt tctcgctgga cgaggtcttc gagatcgcca
    11701 acttcaacaa ctacctgaac cagtcgggca tcaccaagtt caacaccatc atcggcggca
    11761 agttcgtgaa cggcgagaac accaagcgca agggcatcaa cgagtacatc aacctgtaca
    11821 gccagcagat caacgacaag accctgaaga agtacaagat gagcgtcctg ttcaagcaga
    11881 tcctgtccga caccgagtcc aagagcttcg tgatcgacaa gctggaggac gactcggacg
    11941 tcgtgaccac catgcagagc ttctacgagc agatcgccgc cttcaagacc gtcgaggaga
    12001 agtcgatcaa ggagaccctg agcctgctgt tcgacgacct gaaggcccag aagctggacc
    12061 tgtcgaagat ttacttcaag aacgacaagt ccctgaccga cctgtcgcag caggtgttcg
    12121 acgactacag cgtcatcggc accgccgtgc tggagtacat cacccagcag atcgccccga
    12181 agaacctgga caacccctcg aagaaggagc aggagctgat cgccaagaag accgagaagg
    12241 ccaagtacct gagcctggag accatcaagc tggccctgga ggagttcaac aagcaccgcg
    12301 acatcgacaa gcagtgccgc ttcgaggaga tcctggccaa cttcgccgcc atcccgatga
    12361 tcttcgacga gatcgcccag aacaaggaca acctggccca gatcagcatc aagtaccaga
    12421 accagggcaa gaaggacctg ctccaggcca gcgccgagga cgacgtcaag gccatcaagg
    12481 acctgctgga ccagaccaac aacctgctgc acaagctgaa gattttccac atctcccagt
    12541 cggaggacaa ggccaacatc ctggacaagg acgagcactt ctacctggtc ttcgaggagt
    12601 gctacttcga gctggccaac atcgtgccgc tgtacaacaa gatccgcaac tacatcaccc
    12661 agaagcccta ctccgacgag aagttcaagc tgaacttcga gaactcgacc ctggccaacg
    12721 gctgggacaa gaacaaggag cccgacaaca ccgccatcct gttcatcaag gacgacaagt
    12781 actacctggg cgtgatgaac aagaagaaca acaagatttt cgacgacaag gccatcaagg
    12841 agaacaaggg cgagggctac aagaagatcg tctacaagct gctgccgggc gccaacaaga
    12901 tgctgcccaa ggtgttcttc tccgccaagt cgatcaagtt ctacaacccg tccgaggaca
    12961 tcctgcgcat ccgcaaccac tccacccaca ccaagaacgg ctcgccccag aagggctacg
    13021 agaagttcga gttcaacatc gaggactgcc gcaagttcat cgacttctac aagcagagca
    13081 tctccaagca cccggagtgg aaggacttcg gcttccgctt cagcgacacc cagcgctaca
    13141 actccatcga cgagttctac cgcgaggtcg agaaccaggg ctacaagctg accttcgaga
    13201 acatctcgga gagctacatc gacagcgtcg tgaaccaggg caagctgtac ctgttccaga
    13261 tttacaacaa ggacttcagc gcctactcca agggccgccc taacctgcac accctgtact
    13321 ggaaggccct gttcgacgag cgcaacctcc aggacgtcgt gtacaagctg aacggcgagg
    13381 ccgagctgtt ctaccgcaag cagtccatcc cgaagaagat cacccacccc gccaaggagg
    13441 ccatcgccaa caagaacaag gacaacccga agaaggagtc ggtgttcgag tacgacctga
    13501 tcaaggacaa gcgcttcacc gaggacaagt tcttcttcca ctgccccatc accatcaact
    13561 tcaagtcctc gggcgccaac aagttcaacg acgagatcaa cctgctgctg aaggagaagg
    13621 ccaacgacgt ccacatcctg tccatcgacc gcggcgagcg ccacctggct tactacaccc
    13681 tggtggacgg caagggcaac atcatcaagc aggacacctt caacatcatc ggcaacgacc
    13741 gcatgaagac caactaccac gacaagctgg ccgctatcga gaaggaccgc gactccgccc
    13801 gcaaggactg gaagaagatc aacaacatca aggagatgaa ggagggctac ctgtcgcagg
    13861 tcgtgcacga gatcgccaag ctggtcatcg agtacaacgc catcgtcgtg ttcgaggacc
    13921 tgaacttcgg cttcaagcgc ggtcgcttca aggtcgagaa gcaggtgtac cagaagctgg
    13981 agaagatgct gatcgagaag ctgaactacc tggtcttcaa ggacaacgag ttcgacaaga
    14041 ccggcggcgt gctgcgcgct taccagctga ccgccccgtt cgagaccttc aagaagatgg
    14101 gcaagcagac cggcatcatc tactacgtcc ctgccggctt caccagcaag atttgccccg
    14161 tcaccggctt cgtgaaccag ctgtacccca agtacgagtc cgtgtcgaag agccaggagt
    14221 tcttcagcaa gttcgacaag atttgctaca acctggacaa gggctacttc gagttctcct
    14281 tcgactacaa gaacttcggc gacaaggccg ccaagggcaa gtggaccatc gcctcgttcg
    14341 gcagccgcct gatcaacttc cgcaacagcg acaagaacca caactgggac acccgcgagg
    14401 tctacccgac caaggagctg gagaagctgc tgaaggacta ctccatcgag tacggccacg
    14461 gcgagtgcat caaggccgcc atctgcggcg agagcgacaa gaagttcttc gccaagctga
    14521 cctccgtgct gaacaccatc ctccagatgc gcaacagcaa gaccggcacc gagctggact
    14581 acctgatctc ccccgtcgcc gacgtgaacg gcaacttctt cgactcccgc caggctccta
    14641 agaacatgcc tcaggacgct gacgctaacg gcgcttacca catcggcctg aagggcctga
    14701 tgctgctggg ccgcatcaag aacaaccagg agggcaagaa gctgaacctg gtcatcaaga
    14761 acgaggagta cttcgagttc gtgcagaacc gcaacaacag ccgcgccgac ccgaagaaga
    14821 agcgcaaggt gtaggtttaa acgcggacat tcgatttatg ccgttatgac ttccttaaaa
    14881 aagcctttac gaatgaaaga aatggaatta gacttgttat gtagttgatt ctacaatgga
    14941 ttatgattcc tgaacttcaa atccgctgtt cattattaat ctcagctctt cccgtaaagc
    15001 caatgttgaa actattcgta aatgtacctc gttttgcgtg taccttgctt atcacgtgat
    15061 attacatgac ctggacagag ttctgcgcga aagtcataac gtaaatcccg ggcggtaggt
    15121 gcgtcccggg cggaaggtag ttttctcgtc caccccaacg cgtttatcaa cctcaacttt
    15181 caacaaccat catgccacca aaagcgcgta aaacaaagcg agatttgatt gagcaagagg
    15241 gcaggatcca atgcgcgatt caagacatta aaaatggaaa atttcaaaaa attgcgcccg
    15301 cagcgcgtgc atacaaaatt catcccaata caagtgtaat gctagtggag gtcaacacat
    15361 caatgcctat tttggtttag tcgtccaggc ggtgagcaca aaatttgtgt cgtttgacaa
    15421 gatggttcat ttaggcaact ggtcagatca gccccacttg tagcagtagc ggcggcgctc
    15481 gaagtgtgac tcttattagc agacaggaac gaggacatta ttatcatctg ctgcttggtg
    15541 cacgataact tggtgcgttt gtcaagcaag gtaagtggac gacccggtca taccttctta
    15601 agttcgccct tcctcccttt atttcagatt caatctgact tacctattct acccaagcat
    15661 ccaaatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
    15721 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
    15781 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
    15841 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
    15901 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
    15961 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
    16021 ggccgaggag caggactgac cgacgccgac caacaccgcc ggtccgacgc ggcccgacgg
    16081 gtccgaggag cttgagatcc acttaacgtt actgaaatca tcaaacagct tgacgaatct
    16141 ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg tgttcaggat
    16201 ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg atttaatagc
    16261 tccatgtcaa caagaataaa acgcgtttcg ggtttacctc ttccagatac agctcatctg
    16321 caatgcatta atgcattgga cctcgcaacc ctagtacgcc cttcaggctc cggcgaagca
    16381 gaagaatagc ttagcagagt ctattttcat tttcgggaga cgagatcaag cagatcaacg
    16441 gtcgtcaaga gacctacgag actgaggaat ccgctcttgg ctccacgcga ctatatattt
    16501 gtctctaatt gtactttgac atgctcctct tctttactct gatagcttga ctatgaaaat
    16561 tccgtcacca gcccctgggt tcgcaaagat aattgcactg tttcttcctt gaactctcaa
    16621 gcctacagga cacacattca tcgtaggtat aaacctcgaa aatcattcct actaagatgg
    16681 gtatacaata gtaaccatgc atggttgcct agtgaatgct ccgtaacacc caatacgccg
    16741 gccgaaactt ttttacaact ctcctatgag tcgtttaccc agaatgcaca ggtacacttg
    16801 tttag
//